Fruit flies study in home lab

So as we see that to address my research question I need to first trap fruit flies and culture single line/ pure lines by catching gravid females and identify Native Drosophila melanogaster amongst the native fruit flies from the environment!

Following are my research objectives

Immediate objective
To make Home made media for culturing Native Fruit flies.
To create multiple Single Line culture of Native fruit flies by keeping a bait near kitchen garbage using tomato slice/banana peel and catching gravid females and creating Native Single lines.

Short term Objective
To identify native single culture of fruit flies as Native Drosophila Melanogaster by looking at morphological characteristics relevant to Family Drosophilidae, Genus Drosophila and Species melanogaster

Long term objective
To perform olfactory assay at range of concentrations of IAA (banana essense)
using standard CsBz 2nd instar larvae and Native Drosophila melanogaster 2nd instar larvae and compare their olfactory sensitivity to comment on evolutionary changes in terms of fruit odor smelling!

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TRSV - STANDARDIZED
Culturing Fruit Flies At Home Lab

INGREDIENTS
1 egg size Tomato
2 table spoon Rava
1 table spoon Sugar
~150 mL Water (Sanitised bottle)
~15 mL Vinegar (medicine cap)

PREPARATION
Taken a pan turn on flame added ~150ml water ,2 table spoon rava ,1 spoon sugar ,1egg sized tomato smashed . Continually mixed mixture at 10 minutes low flame . Off flame and 5 minutes leave it for cooling .final added ~15ml Vinegar in to whole medium and mixed it well. Look like semi liquid conditions . Directly pure in to well cleaned plastic bottle ~2cm length . Leave it for solidification 24H .

Home Lab :- Bottukadavu Kerala
Date of Media Preparation 4/9/2020 - 5/9/2020

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Locating Observing Fruit Flies

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Fruit Flies are more observed fruits and vegetables specially rotten substances. That’s why the flies are more interested in kitchen places ( Kitchen garbage bin ). If want to observed flies , we can kept any kind of fruits Using as a Bait ( Tomato,Banana ). In this picture one day old tomato piece shows large number of flies [20/08/2020]. Flies are more attracted to rotten substance because of the smell .

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Trapping Fruit Flies

We can trapp fruit flies Using Fruit / Rotten Baits . Bait are 2 type Open bait or Closed bait. Both baits are shows different .in the case of open bait we kept a fruit any kind of paper for attracting flies and in the case of closed bait , fruits are kept in the bottle and invited flies after flies were in to the bottle just closed the mouth of bottle that way we trap flies and culture them safely . In open Bait ( Tomato / Banana ) we can trap flies using an empty bottle inverted . When the flies are sitting over the tomato pieces we can movie closely towards the bait very carefully with inverted bottle then put over the fly . So the where going to upwards because of the behavior is negative giotex . Flies are moving upward direction so we can easily trap/transfer flies like this way .

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Very interesting to see the medium being prepared at home----------when homes turn to labs, magic is invented.

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@saswathy679 This is a very interesting approach! Learnt about this for the first time! :+1:

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HEY, Interesting!! that you said that Fruit flies are more attracted to rotten fruit and vegetables!! or i would add that fruit flies are less attracted to fresh fruits and vegetables, Sooooo can we make a clear Hypothesis and can we test our Hypothesis by an experimental design??? in order to prove this statement!!

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It can seem as if fruit flies come from out of nowhere to infest a home.Fruit flies typically lay their eggs directly on rotting fruits and vegetables or else inside drains that are not kept clean.

Mangoes attracted fruit flies in wide mouth bottle.Objective is to attract fruit flies using different kinds of fruit Bait. Also this picture shows rotten mango pieces are attracted more than flies comparatively fresh mango pieces.

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 Mango Bait
 Bait started date: 10/05/2020.
 Setup time :11:30AM
 Location of Bait : kitchen garbage bin ( sliced mango pieces kept it in a wide mouth plastic bottle )
 Observation : day by day

  1. 10/05/2020 – 0 flies
  2. 11/05/2020 – 1 flies
  3. 12/05/2020 – 10 flies
  4. 13/05/2020 – 14 flies
  5. 14/05/2020 – 50 flies
  6. 17/05/2020 - ~25 above Larvae and Pupae
    Condition - Black spongy things appeared on the above mango pieces. Rot condition.
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Open Tomato Bait -

19 August 2020
Time kept Tomato Bait - 15:29PM
Home Lab Location - Bottukadavu Thrissur Kerala.
Humidity - 25°c
Temperature - 33°c - 26°c
Month - August

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Less Number of Flies

Next Day

20 August 2020
Time kept Tomato Bait - 13:02PM
Home Lab Location - Bottukadavu Thrissur Kerala.
Humidity - 80°c
Temperature - 33°c - 27°c
Month - August

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More Number of Flies

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Definitely. So we can start an experiment with clear hypothesis is Fruit Flies are more attracted rotten fruit comparatively fresh tomato bait. This is my hypothesis and want to prove it’s right or not .

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What Are The Feeding Habits Of Fruit Flies?

As fruit flies feed on decaying fruit, vegetables and other organic items; when living outdoors fruit fly numbers will increase over the summer with their numbers being at their highest during the harvest season. Populations that are found indoors with a consistent food sources will be active year-round. Fruit flies are most active during the day and will feed on the surface of overripe or decaying plant matter. They will also feed on honeydew (secretions) that is produced by aphids.

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