So as we see that to address my research question I need to first trap fruit flies and culture single line/ pure lines by catching gravid females and identify Native Drosophila melanogaster amongst the native fruit flies from the environment!
Following are my research objectives
To make Home made media for culturing Native Fruit flies.
To create multiple Single Line culture of Native fruit flies by keeping a bait near kitchen garbage using tomato slice/banana peel and catching gravid females and creating Native Single lines.
Short term Objective
To identify native single culture of fruit flies as Native Drosophila Melanogaster by looking at morphological characteristics relevant to Family Drosophilidae, Genus Drosophila and Species melanogaster
Long term objective
To perform olfactory assay at range of concentrations of IAA (banana essense)
using standard CsBz 2nd instar larvae and Native Drosophila melanogaster 2nd instar larvae and compare their olfactory sensitivity to comment on evolutionary changes in terms of fruit odor smelling!