Fruit flies observed on a slice of Banana. Doing some kinds of activities like Feeding banana, Walking over there, Shows courtship behavior (If a female fruit fly is receptive to mating, she responds to male pheromones and courtship songs by engaging in a behavior), Some of them taking rest on the bait. Date of observation 22 march 2021 - 11:07am. Location - Kitchen out side.
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Banana Bait 
22 March 2021
Banana Bait Kept Time - 11:00AM
Banana Kept Place - Kitchen Out Side
Humidity - 64%
Temperature - 37 / 25 °C
Month - March (Summer Season)
Observation - 11:07 Observed 10 More Flies Present Over the Banana Slice.
Home Lab - Bottukadavu Thrissur Kerala.
Picture Credit - @saswathy679 
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What do you plan further with the fruitflies that you have observed?
Did you greet them just for a one-time visit or you are planning to trap them?
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But why do you want to trap the fly?
What are the objectives?
I remember that you had three-four single line culture series that were the first one in the CUBE HomeLab Movement prepared with the TRSV Medium.
What happened to them? Could you elaborate on them?
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Iam trying to find out drosophila melanogaster from around my native area. So that’s why Iam making more single line culture using one gravid fly. After getting progeny’s of that species tried to identified them looking at their morphological characters (Head, Wing, Thorax, Abdomen).
Currently I have 4 single line cultures in my home lab, also I tried to identified them at Family - Drosophildae ,Genus - Drosophila level. Making more single line cultures will help us to identification process will become easier, we can compare both different single cultures flies body features, even we will get similar species flies (e.g Drosophila melanogaster - wild one) from native place.
SINGLE LINE CULTURES
Cube Home Lab - Bottukadavu Thrissur Kerala.
SLBK A
Single Line Bottukadavu Kerala
Mother : A fly
Single line culture started on : 26/05/2020
Stock culture (e.g) : A10 (maintain A species flies in stock culture bottles, that will help us to keep that fly progeny’s without loosing)
Stock culture made on : 13/12/2020
Media : TRSV, made on : 12/12/2020
A Progeny’s : ~70 more flies.
Larvae : ~50 larvae.
Pupae : ~100 hatched & un hatched pupae.
Dead flies : ~40 more
SLBK A10 is shows a healthy bottle 
.
31 Days old culture bottle
Video taken : 14/01/2021
Video captured by : @saswathy679
SLBK B
Single Line Bottukadavu Kerala
Mother : B fly
Single line culture started on : 26/05/2020
Stock culture (e.g) : B10 (maintain B species flies in stock culture bottles, that will help us to keep that fly progeny’s without loosing)
Stock culture made on : 13/12/2020
Media : TRSV, made on : 12/12/2020
A Progeny’s : ~100 more flies.
Larvae : ~80 larvae.
Pupae : ~100 hatched & un hatched pupae.
Dead flies : ~50 more
SLBK B10 is shows a healthy bottle .
41 Days old culture bottle
Video taken : 23/01/2021
Video captured by : @saswathy679
SLBK C
Single Line Bottukadavu Kerala
Mother : C fly
Single line culture started on : 11/06/2020
Stock culture (e.g) : C10 (maintain C species flies in stock culture bottles, that will help us to keep that fly progeny’s without loosing)
Stock culture made on : 13/12/2020
Media : TRSV, made on : 12/12/2020
A Progeny’s : ~90 more flies.
Larvae : ~60 larvae.
Pupae : ~90 hatched & un hatched pupae.
Dead flies : ~30 more
SLBK C10 is shows a healthy bottle .
41 Days old culture bottle
Video taken : 23/01/2021
Video captured by : @saswathy679
SLBK E
Single Line Bottukadavu Kerala
Mother : E fly
Single line culture started on : 20/08/2020
Stock culture (e.g) : E10 (maintain E species flies in stock culture bottles, that will help us to keep that fly progeny’s without loosing)
Stock culture made on : 13/12/2020
Media : TRSV, made on : 12/12/2020
A Progeny’s : ~80 more flies.
Larvae : ~60 larvae.
Pupae : ~100 hatched & unhatched pupae.
Dead flies : ~30 more
SLBK E10 is shows a healthy bottle .
41 Days old culture bottle
Video taken : 23/01/2021
Video captured by : @saswathy679
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IDENTIFICATION OF FRUIT FLY
To Identify Single Line Culture Fruit Flies Looking Their Morphological Characters.
Objective is to identify native drosophila melanogaster in my locality area.
Fruit flies are a very small oval fly with adults growing only to be 3mm length. Their thorax is tan in color and their abdomen is black with a gray underbelly, fruit flies , however tend to have an overall tan appearance. Fruit flies often have red eyes, although some can have dark colored eyes (Phorid fly)
Reference 
: Fruit Flies - Types, Facts, and How to Identify | Fruit Fly Infestation | Holder's Pest Solutions.
Fruit flies which are belongings to,
Kingdom:Animalia
Phylum:Arthropoda
Class:Insecta
Order:Diptera
Family:Drosophilidae
Genus:Drosophila
The Order Diptera (true flies) includes many common insects such as mosquitoes, midges, sand flies, blowflies and the House Fly, Fruit Fly.
Most of the insects we see flying around do so with four wings (two pairs), but dipterans (meaning ‘two wings’) use only one pair & 3 pair of legs which are present in their body.
Characteristics of the order - Diptera
• One pair of wings (forewings)
• 3 pair of legs
• A large and moveable head
• Compound eyes that are often very large
• Sucking, piercing and sucking or sponge-like mouthparts (all adapted for a liquid diet).
Referred from Morphology of Diptera - Wikipedia
Pictures captured by @saswathy679
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Where can we find fruit flies 
!!?..
Fruit flies are especially attracted to ripened Fruits and vegetables in the kitchen. But they also will breed in drains, garbage disposals, empty bottles and cans, trash containers, mops and cleaning rags. All that is needed for development is a moist film of fermenting material.
Fruit Flies observed in ;
1)
Banana pieces
2)
Orange peel
3)
Stoberry pieces
4)
Coconut
5)
Winter Lemon
6)
Lemon
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How can we culture fruit flies at home 
? Is it possible!!!
Why not 
. We can culture flies using ingredients which are available at our home, specially in our kitchen. So, in here we are making a good healthy halva for for fruit flies 
.
1st step - Cleaning process
Before media preparation we have to look very important thing, that first one is that ;Cleaning bottles.
Collect same sized any bottles (~100mL,250mL,500mL bottles)
Clean- Washed it well using detergents & Leave it under the sun for drying. After dry bottles take in to inside. Keep it safe place.
2nd step - Media preparation
Media for culturing fruit flies - making TRSV media. Planing to make ~100 media (~100mL) bottles.
Ingredients :
- Tomato - 1 egg size
- Rava - 2 table spoon
- Sugar - 1 table spoon
- White Vinegar - ~15mL
- Water - 150mL
Preparation :
•Take a pan add on lower to medium flame. Add150mL water measured using 100 mL bottle (Apple fizz), 2 table spoon rava, 1 table spoon sugar, 1 egg size tomato chopped add, mixed it continuously till 10minutes. After turn off flame. Than put that pan under fan for avoiding heat. After 3 minutes later add ~15mL White vinegar - 4% acetic acid (measured by medicine cap).Mixed it well. Look like dosa batter we can able to transfer well cleaned bottles.
3rd step - Set a Trap
Using any kind of fruit & vegetables put a trap for trapping fruit flies.
4th step - Catching Flies
Take a empty plastic bottle keep in your hand inverted condition. Slowly closer to the bait and trap them. Why we using this method because Fruit flies have a positive geotaxis behavior. After enter flies will not escape.
5th step - Transfer flies
Transferring this flies in to media bottle using this method.we can easily to maintain them this way.
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ACTIVITIES PATTERN OF FRUIT FLIES
Experiment : To check the activities of fruit flies during day and night time.
Do fruit flies sleep at night!!?
Design of the experiment : Checked flies culture 4 bottles, starting from morning to night to check what kind of activities doing inside bottles like mating, feeding, courtship, walking, egglaying, flying, etc … want to provide/disproved do flies sleep at night!
Set up :
- 4 Fruit flies freshly made culture bottles taken for this experiment
- 6 days old culture bottles,
- Most of the flies newly emerged flies (15/04/2021).
- Keep this bottles inside home (Hall). There was light not normal conditions.When was taking videos and photos, only that time was on the light in there fot 2sec
Experiment : Taken 5 set videos of that culture bottles at morning & night. Totally ~100 flies (A= ~30, B= ~20, C= ~25, E= ~20)
Observation : Day 1st
Morning :-
7:00am - 20% active
7:30am - 50% active (flying + walking)/ 50% not active
8:00am - 50% active (feeding + walking + flying)
8:30am - 80% active (feeding + walking + flying + courtship behavior)
9:00am - 100% active (feeding + walking + flying + courtship behavior).
Video, Time of the video:9.30am, Date: 21st April 2021
Video, Time of the video:11.30am, Date: 21st April 2021
Night :-
09:30pm - 50% not active + 50% active
10:00pm - 75% not active + 25% active (walking + flying + playing under cotton plug)
10:30pm - 80% not active + 20% active (walking + flying)
11:00pm - 90% not active + 10% active (walking, but not continuously)
11:30pm - 100% not active.
Video Time of the video: 11.41pm, Date:21st April 2021
Goof Up : I didn’t take continues vidoes ,that make graph became inperfect. almost light is effcting the activities of fruit flies.
Planing to take videos continuously 4 days that will help to get a rhythmic pattern. That will help us to plotting graph.
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Activities Pattern Of Fruit Flies
Study inside fruit flies cultures bottles
[center]Expectation - Graph[/center]
06:00am - 0% not active
07:00am - 20% active
08:00am - 40% active
09:00am - 60% active
10:00am - 70% active
11:00am - 80% active
12:00pm - 100% active
01:00pm - 85% active
02:00pm - 80% active
03:00pm - 60% active
04:00pm - 40% active
05:00pm - 20% active
06:00pm - 0% not active
{Activities ; Feeding, Mating, Courtship, Egglaying, Walking, Flying}
I am expecting flies activities will increasing after sunrise & get one peak afternoon 12:00pm. After sunset flies activity will decrease slowly.
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Activities Pattern Of Fruit Flies
[Studying inside fruit flies cultures bottles]
[center]Day 1- Graph[/center]
1).Starting from 7:00am (10%) flies staring active.
2).100% flies active starting from 10:00am.
3).0% activities observed 12:00am till 6:00am
Flies bottles kept inside home & also other LED lights effect the observation. Can see the variations coming in activity pattern of fruit flies during day & night.
[center]Day 2 - Graph[/center]
Objective : To study activity pattern of fruit flies (6:00am - 12:00am) inside culture bottles.
Hypothesis : Flies are active during day time and sleep at during night time (certain time) inside culture. We will get a pattern that similar to observed in circadian rhythm study. There is internal clock controlling fruit flies activities day & night.
Number of samples : 4 Bottles newly transferred flies taken for this activity pattern study. (SLBK A15, SLBK B15, SLBK C15, SLBK E10 - 16/04/2021)… In 4 bottles total ~95 flies.
Method of data collection : starting from 6:00am (1 hour) interval taking only 12 sec videos till when flies will completely not active time.
Observation :
•Flies not active 6:00am, 11:00pm (these two time observed flies were not active)
•Flies stated active morning 7:00am (10%)
•Flies (100%) active 11:00am, 12:00pm
Conclusion : Fruit flies active slowly starting from 7:00am till 7:00pm after this flies showing slightly variation came in %.Flies do rest at night (11:00pm - 6:30am) this time observed most flies were in active conditions.
Further plan : Take videos continuously 4 days. To get a proper data.
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[center]Night Time Activities of Fruit Flies[/center][center]21st April 2021[/center]
Video at 9:30pm - activity pattern of fruit flies [21/04/21]
Video at 10:00pm - activity pattern of fruit flies [21/04/21]
Video at 10:30pm - activity pattern of fruit flies [21/04/21]
Video at 11:00pm - activity pattern of fruit flies [21/04/21]
Video at 11:30pm - activity pattern of fruit flies [21/04/21]
9:30pm,10:00pm,10:30pm,11:00pm,11:30pm - 5set (5sec videos). From 9:30pm flies slowly starting to resting, we can see 50% at 9:30pm,25% at 10:00pm,20% at 10:30pm,10% at 11:00pm, 0% at 11:30pm - observe slowly decreasing activities of fruit flies night time.
[center]Graph[/center]
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Tell us the inference of the observations made so far (in the form of video)!
What is seen in the videos?
More of us would be excited in knowing what is happening inside the bottle after which the video can be seen.
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@drishtantmkawale, this was showing the activities of fruit flies inside culture bottles. What Iam tried to taken videos that the end point of sleep. my purpose is doing this experiment to find when will fruit flies completely taking rest condition!?. here is 5 set videos started from 9:30pm onwards we can able to see most of flies were are not active conditions. 10:30pm&11:00pm Fruit flies are completely not active.11:30pm showing that flies were end point of rest, not much movement couldn’t able to observe in 4 culture bottle flies.almost this experiment provide that fruit flies sleeping during night time…!
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[center]Day Time Activities of Fruit Flies[/center][center]22nd April 2020[/center]
Video at 7:00am - activity pattern of fruit flies [ 22/04/2021]
Video at 7:30am - activity pattern of fruit flies [ 22/04/2021]
Video at 8:00am - activity pattern of fruit flies [ 22/04/2021]
Video at 8:30am - activity pattern of fruit flies [ 22/04/2021]
Video at 9:00am - activity pattern of fruit flies [ 22/04/2021]\
7:00am,7:30am,8:00am,8:30am,9:00am - 5set (5sec videos). From 7:00am flies slowly starting to activities we can see 20% at 7:00am,50% at 7:30am,50% at 8:00am,80% at 8:30am, 100% at 9:00am - observe slowly increasing activities of fruit flies day time.
[center]Graph[/center]
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[center]Camparing day 
& night 
activities of fruit flies inside culture bottles…![/center]
10:00am video _
10:00pm video _
11:00am video _
11:00pm video _
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Are fruit flies seasonal 
?
Fruit flies can be a problem year round, but are especially common during late summer/fall because they are attracted to ripened or fermenting fruits and vegetables. Fruit flies are also attracted to rotting mango, bananas, potatoes, onions and other unrefrigerated produce purchased at the grocery store.
Fruit flies are common household pests that can be a terrible nuisance around our home. These flies are year-round pests, but they are especially prolific in late summer and fall.
Fruit flies move into kitchens, hall, window and basements if they sense a food source. Overripe fruit on the counter or any fermenting matter in drains, mops, and trash bins appeals to them. Unsuspecting homeowners may also bring these pests inside on garden crops.
Fruit flies are especially attracted to ripened fruits and vegetables in the kitchen. But they also will breed in drains, garbage disposals, empty bottles and cans, trash containers, mops and cleaning rags. All that is needed for development is a moist film of fermenting material.
Where do fruit flies come from in the winter?
Adult fruit flies lay eggs in rotting fruit and plant material. They may live in and around your garbage or drain. Cluster flies are common around windows, attics, and basements. They look for warm, secluded places where they can huddle together and enter diapause.
Seasonal Abundance of Mango Fruit Flies (Diptera:Tephritidae) and Ecological Implications for Their Management in Mango and Cashew Orchards in Benin (Centre & North)
We report study on seasonal population fluctuations of fruit flies (Dipteran Tephritidae) in mango (2005–2009) and cashew (2007–2009) orchards in the Borgou Department, Benin. During the five consecutive years of mango fruit fly. The most abundant species during the dry season, peaked beginning of May.
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[center]Feeding Habits Drosophila[/center]
Video: 1
Video: 2
Video - 3
Fruit flies were observed rotten ripe Mangoes 
.During the preoviposition feeding stage, adult fruit flies spend time feeding on fruits, vegetables and other decaying materials.
Photo taken :Aswathy suresh.
Location 
:Bottukadavu-Thrissur Kerala.
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