But will those colonies be free of any waste?
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Yes obviouslyā¦when streaked we get isolated colonies of any bacteria (if streaking is done well).how can waste grow ? @pratittodkar
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Itās simpleā¦once you get isolated colonies we can compare the colony characteristics and can conclude whether the colonies are of lactobacilli are not .
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I donāt mean grow in the sense living things do I mean pile up. The colonies will obviously produce some waste right? and if it is significant in comparison with the waste from milk then this will be of no use.
@Sjuday2527 could this be the reason for the dead moina yesterday? Since the distilled water was kept in blue vessel and when you went to take it they got sudden exposure to blue light.
So, according to you, what are the lactobacilli colony characteristics?
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@drishtantmkawale Please state your immediate term, short term and long term objectives?
How are we planning to achieve those in moina work?
Are you also planning to identify some bacteria produced in moina cultures? Please state the reasons behind it, and how will you be proceeding to do it?
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Ok so even if the colony is producing any waste still itās so less as compared to the milk we are putting in the bottle cultures. Donāt you think this would help us to save moina cultures for much longer period of time?
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Lactobacilli colonial morphology can vary from small to medium gray colonies that usually exhibit alpha hemolysis on blood agar. Lactobacilli grow on a variety of other media including MRS (Man, Rogosa, and Sharpe) agar where they appear as white, usually mucoid colonies.
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I donāt know the first thing about culturing bacteria so if what you say is the case then that would be great for our cultures.
@Sjuday2527 I didnāt got any input from you on this one. I would love to know what do you think about it?
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Well , apologize me as I forgot to post it ā¦
As every day I was collecting moinas from bottles for feeding Hydras. As to give a wash to the moinas collected in a plastic cup I had put DC water from a blue colour bucket. And suddenly what I observed was in less than a minute all the moinas which I collected in the plastic cup died which were all alive just a minute backā¦I was completely amazed 
So now after @pratittodkar stating about the blue light, now I got to know that the moinas which I assumed to be dead were alive but paralyzed.
But my question is why ??? How is change in color of light bringing such a drastic and such an extremely quick change ? How does paralysis occur ? A great topic to discuss on ā¦ @pratittodkar @drishtantmkawale please pitch in. ā¦your inputs too will help such topics interesting and clarifying.
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I should clarify that I am just hypothesizing that it may be the case though the above reference is from a paper and is completely legit but we should do a test and see how much time does it require to Moina get paralyzed. The paper dosenāt say anything about the time required.
Ya we should do it. Most probably on next Monday tell me how early should I be there so that we can get maximum time for discussing the matter and perhaps doing something in that direction. This Monday I came late and @Sjuday2527 had to leave by then so I donāt want that to happen next monday again. If I am early I can stay there until evening 6 or 7 and hence I can get more time.
That was a great observation!! 
@Sjuday2527
So, I searched on Google for āeffect of light on Daphniaā and there were many assays performed with different light intensities on daphnia and all have almost the same result!!
Either the heartbeat is increasing or decreasing.
Theyāve used Red, Blue and White lights.
Iām dropping down some of the linksā¦
Do look out.
I have the procedure for this assay, if required, Iāll attach it laterā¦
1_93.pdf (1.0 MB)
Motion control of Daphnia by Blue LED light, Tokyo Denki University, Japan
J2217.pdf (13.9 KB)
Can light Control heart rate? By Harsha Thangavijayan - California state Science fairā¦ Seems Indian origin though 
I think these articles will provide us starter for our designā¦ which we should do at the earliestā¦!!
What say? @Sjuday2527 @pratittodkar
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Greaatttt @drishtantmkawale this is why I told and mentioned you both because together we can solve many questions. Just from a single question of how to culture moina in the start , now we have reached at a level where we are talking about the different tests to be performed to check water quality and detect and measure different chemical compounds, checking effects of different wavelengths of light on moina , checking whether it can survive in distilled and rain water or not and seriously many many more things, complicated things like kinetics of moina growth and using colormiter without any high-tech instrumentaion on our phone itselfā¦ Amazing work by u both @drishtantmkawale and @pratittodkar learned literally a lotttt because of you allā¦thanks buddies 
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Can we measure the heartbeat of moina in our lab ? If yes then we too can perform effects of different wavelengths of lights and check their effects on moina.
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By this we can also know how does paralysis occur in moina ? And can this be reversed tooā¦
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Letās see whether we are able to manage time or not.
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