S4E150 CUBE chatShaala: Cardamine: context to understand Gene expression and KNOXX gene

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Summary of discussion quoted by @saswathy679
White Boards Of Yesterday Chatshaala : 19.08.2021
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Chai & Why session in 29th August 2021. Making posters including home lab experiments. Can we setup a lab at our home :thinking:? Can the science at home be as good as the science in lab!

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What is the exact process of gene expression in cells :thinking:? Before that we need to know what is Gene? Where can gene present inside nucleus!.
Tryingto find out this kind of fundamental questions answers through communication.!
How to introduce model systems in a simple way that every people can understand, get inspired and do some works on it. Specially who are join in Chai & Why section, even non biologists.

Today we were discussed about model systems.
:black_small_square: Phyllanthus
:black_small_square: Nematodes

But we covered other areas like Butterfly - Plant interaction, Cardamine (Gene expression).

Lakshmi @Shraddha Adithya mentioned about Journey about when did started doing work in CUBE.

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Gene Expression in Cardamine plant.
KNOX Genes : Compound leaves formation because of KNOX genes expression.
Simple leaves change in to compound leaves.

One of other Gene expression example is Moina. Body color change in White to Red. This condition called Hypoxia Condition. Happen in low oxygen levels (Unfavorable conditions)
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Cardamine Leaves stage study
Research Q : Floral dip method in Cardamine flowering bud.
Which leaves stage cardamine flowering bud will form in order to study floral dip method.
4-6 stages of leaves were showing flowering. Total 45 to 50 days taken for to get first floral bud in cardamine plants.
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Story of Butterfly’s studies done in HBCSE Campus (Butterfly - Plants interaction study done by cubits Kiran @drishtantmkawale @Lydiamathew Adithya…& others)
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Summary of discussion quoted by @Shraddha276

Summary of CUBE Chatshaala (19/8/21)
We started discussion on Chai & Why session.
✓What is Chai & Why?
✓ Introduction of Chai & Why.
✓How to introduce all model systems.

So we started discussion on C.elegans & Phyllanthus.

In C.elegans model organism - we discussed how to connect Round worm in stomach with Nobel prize winning work & with Isolating of C.elegans on potato slices.

In Phyllanthus model organism - we discussed about how to connect Nobel prize winning work in fruit flies with Continuous light & dark experiment in Phyllanthus. also leaf angle titration connect with acid base titration.
Also highlighted on Phyllanthus niruri controversy.

Today’s discussion was on 4 different plants -
1.Mimosa
2. Phyllanthus
3.Cardamine
4.Mango mapping

In Phyllanthus we discussed about
@⁨Lakshmy PJ CUBE⁩’s story of Phyllanthus-
Initially @⁨Lakshmy PJ CUBE⁩ was working on Mimosa plants for Circadian rhythm study.she also done seed germination study in Mimosa plant.
Then she working on Phyllanthus for Circadian rhythm study, Identification of Phyllanthus species,leaf angle titration.
Collaborators- @⁨P. Chitralekha Cube JNU⁩ mam @Nidhi @Anushka Datta @ Anulekha @Shraddha

Then we discussed on my (Shraddha) story of Phyllanthus. I found some plants in plants pot at home.
I got interested because of Circadian rhythm study was in my MSc syllabus & because of observations done by @Harshad sir,in CUBE HBCSE.
Great observations done by Susanta Tanti Sir & their students while returning to Assam from Mumbai by train.
They were observed how many Mango trees was flowering & then they mapped it.

Then we discussed on Cardamine plant-

• Lifecycle of Psyche butterflies on Cardamine plant were observed in G6 lab HBCSE.

• how many days required to get first flower buds?

• after how many Compound leaves first flower buds arose?

• why Cardamine having compound leaves & Arabidopsis having simple leaves?

Which genes responsible for leaf diversity in both?

•how do gene expressed?so we compare Cardamine- plant model organism with Moina

While understanding gene expression questions was arise-
1.what is a gene?
2.where it’s located inside the cell?
3.how gene expressed?
4.why Chromosomes are important in cell?
5.what is Chromosomes & Chromatin?
@⁨Seethalakshmy CUBE⁩ @⁨Aswathy Suresh CUBE⁩ @⁨Aditiya Joshi CUBE⁩ @⁨Lakshmy PJ CUBE⁩ @⁨Aastha Ahuja Cube⁩ @Mandar

Yesterday Discussion Based Reference Given in CHATBOX.

Analysis of circadian leaf movement rhythms in Arabidopsis thaliana Arabidopsis thaliana is the model organism for the study of the higher plant circadian clock. The physiological change in position of young leaves and cotyledons in Arabidopsis seedlings reveals an overt circadian rhythm.
https://pubmed.ncbi.nlm.nih.gov/17417004/#:~:text=The%20physiological%20change%20in%20position%20of%20young%20leaves,requires%20no%20prior%20genetic%20manipulation%20of%20the%20plant.

Although Arabidopsis lacks the pulvinus, it displays rhythms in cell elongation and, thus, in growth rate. For example, there is a circadian rhythm in the elongation rate of the abaxial and adaxial cells of the petiole that confers an oscillation in position of cotyledons and leaves (Engelmann and Johnsson, (1998)).https://www.google.com/search?q=circadian+rhythm+Arabidopsis&sxsrf=ALeKk01jaHuqFRhL7pTvNjqTLuhT1tPRRA%3A1629377521467&ei=8VMeYfToG9iPxc8P6fmeqAU&oq=circadian+rhythm+Arabidopsis&gs_lcp=Cgdnd3Mtd2l6EAMyBQgAEIAEOgcIABBHELADOgcIABCwAxBDOgoIABCwAxBDEIsDOgsIABCABBCxAxCDAToECAAQQzoKCAAQsQMQgwEQQzoGCAAQFhAeSgUIPBIBMUoECEEYAVDFywVYwuwFYNXzBWgBcAB4AIAB3wKIAaYckgEGMi0xMC4zmAEAoAEByAEKuAECwAEB&sclient=gws-wiz&ved=0ahUKEwi0pfyikL3yAhXYR_EDHem8B1UQ4dUDCA4&uact=5

https://pubmed.ncbi.nlm.nih.gov/17417004/#:~:text=The%20physiological%20change%20in%20position%20of%20young%20leaves,requires%20no%20prior%20genetic%20manipulation%20of%20the%20plant.
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana

https://www.semanticscholar.org/paper/KNOX-genes%3A-versatile-regulators-of-plant-and-Hay-Tsiantis/3a4eaa60fa0951d978689e116fbb1dd4004738cf/figure/7
Interesting picture on Knox gene

A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsutashow that lateral leaflet formation in C. hirsuta requires the establishment of growth foci that form after leaf initiation. https://pubmed.ncbi.nlm.nih.gov/19165928/

Class I KNOTTED1-like homeobox (KNOX) proteins also promote leaflet initiation in C. hirsuta, and here we provide evidence that this action of KNOX proteins is contingent on the ability to organize auxin maxima via the PINFORMED1 (PIN1) auxin efflux transporter