Is it not wonderful that we can isolate nematodes from soil samples at Home? This is one of the innovations that we made during lockdown. To achieve this method we first went through the understanding of our standard lab based method of isolation of soil nematodes and particularly finding of C. elegans from India.
This is the standard lab based (specific amount of media and a known feed) method of isolation of soil nematodes where soil is placed at the circumference of extraction plate:2%Agar and E.coli culture at the center of the plate to attract nematodes from soil to the center so that you can track the movement of nematodes and take them out from this plate.
Can you think of any alternative to the agar medium and E.coli culture so that we can do the same at home? I encourage you to follow this.
“Whatever things are used, try to break it down to what is its function and can it be replaced by things available at home”.
Aim of the experiment and set up :To isolate nematodes from soil on raw potato surface using two drops of milk at the Centre. Soil from a roadside plant has been taken. The soil is kept moist by adding a drop of water
BREAKING NEWS
13 Days after observed Microworms growing well in New separately made TRSV media bottle…Made bottle on 28/05/2021… Transferred some microworms from old culture media bottle to new media bottle using brush… Today observed some amount of microworms crawling bottle media side…
Update by : Aswathy Suresh CUBE SN Nattika.
Date of update : 9.6.2021
Bottle 1 (Microworm culture)…observation…Huge number of worms growing inside media bottle… Only 1 setups I observed microworm growing well conditions… Bottle 2 can’t observed any microworms till today.
SLBK A17 (8/05/2021)…subculture bottle of A species… worms are growing media well…observed some number of worm growing wall of these bottle side after 1 months old culture bottle.
Update : 9.6.2021
Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
SLBK C17 (8/05/2021)…subculture bottle of C species…worms were growing well inside these bottle 1 month old sub culture bottle…
Update : 9.6.2021
Update by : Aswathy Suresh CUBE SN Nattika.
Home Lab : Bottukadavu - Thrissur Kerala.
